学习及工作简历：
1999.9-2003.7 烟台大学 化学生物理工学院 学士
2003.9-2008.6 四川大学 化学学院 硕、博士
2008.7-2011.6 四川大学 化学学院 讲师
2011.7-2016.8 四川大学 化学学院 副教授
2016.9-至今 四川大学 化学学院 教授
主要研究方向：
荧光染料构建；细胞成像；活体成像；荧光手术导航；光动力治疗
在研科研项目：
1.国家自然科学基金区域创新发展联合基金（U25A20592），呫吨类近红外II区荧光染料的智能构建及在肝切除手术中的应用示范，项目负责人，2026.01-2029.12
2.国家自然科学基金面上项目（22077088），近红外II区荧光染料的构建及其在骨关节炎诊疗中的应用研究，项目负责人，2026.01-2029.12
主要奖励和荣誉：
1.宝钢优秀教师奖（2025）
2.英国皇家化学会会士（FRSC, 2024.09）
3.第十五批四川省学术和技术带头人 （2023.07）
4.四川省“天府青城计划-天府科技菁英（2020.12）
5.基于联萘酚结构的荧光探针，四川省科技进步奖（自然科学类）二等奖（2019, 3/3）
6.四川省国际大学生创新大赛优秀指导教师 （2024.11，省金、国银项目）
7.四川省国际“互联网+”大学生创新创业大赛 优秀创新创业指导教师（2020.11，省金、国银项目）
8.第五届全国大学生化学实验创新设计大赛总决赛 特等奖 指导教师（2024.12）
主要工作业绩：
长期致力于荧光染料的设计、智能化分子探针的构建及在肿瘤等重大疾病诊断与治疗方面的应用基础研究。先后主持国家重点研发计划课题、国家自然科学基金（区域创新发展基金、面上项目等）及四川省杰出青年科技人才基金在内的国家和省部级项目8项，以第一或通讯作者在 J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater. 等杂志上发表学术论文120余篇，他引6000多次，获准国家发明专利15项。获得四川省科技进步奖（自然科学类）二等奖1项（排名第三），广东省科技进步奖二等奖1项（排名第四）。现担任中国化工学会精细化工专业委员会委员、中国感光学会-光学传感与诊疗专业委员会委员，四川省化学化工学会分析化学专业委员会副主任委员。
近五年发表论文：
1. Xie, K.-P.; Zhang, H.; Chen, J.; Shi, L.; Zhao, Y.; Bai, L.; Li, K.*, A PET-Driven Strategy for Ultrasensitive Mapping of Lipid Microenvironment Heterogeneity in Dual Organelles during Metabolic Stress and Atherosclerosis. J. Am. Chem. Soc. 2026, 148, 1233–1246.
2. Zhang, H.; Wang, X.-Z.; Zhou, D.-H.; Debnath, S.; Xiang, F.-F.; Kim, D.; Wu, M.; Kim, S.; Li, K.*; Kim, J.-S.*. A lifetime window into lipid dynamics in Alzheimer’s disease. Mater. Today, 2026, 96, 103293
3. Xia, W.-L.; Ran, X.-Y.; Zhao, Y.; Zhang, H.; Zhou, Q.; Yu, X.-Q.*; Li, K.* Fine-Tuning J-Aggregation via Steric Hindrance for High-Efficiency NIR-II Bioimaging and Photothermal Therapy. Mater. Horiz., 2026, 13, 2385-2397.
4. Zeng, X.; Yu, K.-K. Zhang, H.; Zhao, Y.; Wang, A.; Wang, X.; Wu, M.-Y.; Yu, X.-Q.; Chen, S.-Y.*; Li, K.* NADH-Triggered Photogenerated Carbon Radicals as General Abstracting Agents for Synergism of Apoptosis and Ferroptosis. JACS Au. 2026, 6, 268-278
5. Zhang, R.-R.; Ran, X.-Y.; Yu, K.-K.; Zhao, Y.; Zhang, L.-N.; Lv, X.-F.; Zhang, H.; Yu, X.-Q.; Li, K.* Rational Design of NIR-II Fluorescence/Photoacoustic Nanosensor Tailored for Mechanisms of Diabetes-Related Breast Cancer. Adv. Mater. 2025, 37, 2415891.
6. Zhang, H.; Xiang, F-F.; Zhou, D-H.; Liu, Y-H.; Wang, Z-Y.; Chen, S-Y.; Yu, X-Q.*; Li, K.* Precise molecular spacer engineering for tumor-responsive pKa tuning: a pan-cancer imaging platform for evaluating tumor microenvironment reprogramming capability. Sci. Bull. 2025, 70, 4219-4229.
7. Zhou, D.-H.; Chen, S.-Y.; Liu, Y.-Z.; Zhang, H.; Li, J.-M.; Zhou, Q.; Xiang, F.-F.; Yu, X.-Q.; Li, K.*. Thioketal-photocage: a universal modification strategy for constructing new photochemical tools for real-time imaging in living cells. Sci. Bull. 2025, 70, 1087-1096.
8. Xia, W.-L.; Ran, X.-Y.; Xie, K.-P.; Zhao, Y.;Chen, J.; Zhou, Q.; Yu, X.-Q.; Li, K.* Optimized Indocyanine Green Nanopreparations for Biomedical Applications. Coordin. Chem. Rev. 2025, 528, 216422.
9. Zhang, L.-N.; Wei, Y.-F.; Ran, X.-Y.; Shi, L.; Wang, Z.-Y.; Chen, S.-Y.; Yi, C.; Yu, X.-Q.*; Li, K. * Liberating Exciton Transfer as a Pioneering Strategy to Construct Type I Photosensitizers for Hypoxia Deep-Seated Metastases Suppression. JACS Au. 2025, 5, 3982-3993.
10. Zhang, L.-N.; Chen, S.-Y.* Shi, L.; Ran, X.-Y.; Zhang, H.; Moon, H.; Lee, Y.; Yu, X.-Q.; Kim, J. S.*; Li, K.* De Novo Construction of pKa-Tunable Xanthene Molecules for pH Sensitive Fluorescence Navigation. Adv. Funct. Mater. 2025, 35, 2412595.
11. Zhang, L.-N.; Ran; X.-Y.; Zhang, H.; Zhao, Y.; Zhou, Q.; Chen, S.-Y.*; Yang, C.; Yu, X.-Q.; Li, K. * Molecular Engineering of Xanthene Dyes with 3D Multimodal-Imaging Ability to Guide Photothermal Therapy. Adv. Healthcare Mater. 2025, 14, 2402295.
12. Zhao, Y.; Zhang, R.-R.; Wang, N.; Tian, X.-L.; Zhang, L.-N.; Xia, W.-L.; Wang, Z.-Y.; Yu, X.-Q.*; Li, K. * π-Bridge engineering strategy: tailoring S-xanthene dyes with strong absorption for high-efficiency photothermal therapy. Mater. Horiz., 2025, 12, 10698-10708.
13. Xiang, F.-F.; Zhang, H.; Wu, Y.-L.; Chen, Y.-J.; Liu, Y. Z.; Chen, S.-Y.; Guo, Y.-Z.; Yu, X.-Q.; Li, K.* Machine Learning-assisted Rational Design of Si-rhodamine as Cathepsin-pH Activated Probe for Accurate Fluorescence Navigation. Adv. Mater. 2024, 34, 2404828
14. Ding, R.-L.; Liu, X.; Zhang, W.-J.; Chen, X.; Chen, S.-Y.; Yu, X.-Q; Zhao, Z.-H*; Li, K.* Purine-Based Ir(III) Photosensitizers for Efficient Treatment of Periodontal Inflammation, Adv. Funct. Mater. 2024, 34, 2405499
15. Chen, X.; Shi, L.; Ran, X.-Y.; Xu, J.-X.; Zhang, L.-N.; Kong, Q. -Q; Yu, X.-Q.; Li, K.* Molecular Engineering of D-𝝅-A Conjugate with N-Heterocycle Purine for Enhanced ROS Generation and Photodynamic Therapy, Adv. Funct. Mater. 2024, 34, 2400728.
16. Ran, X.-Y.; Xia, W.-L.; Zhang, L.-N.; Yu, X.-Q.; Chen, P.; Xie, K.-P.; Zhao, Y.; Yi C.; Li, Kun* De novo design of type-l photosensitizer agents based on structure-inherent low triplet energy for hypoxia photodynamic therapy. Mater. Horiz., 2024, 11, 5589-5599.
17. Chen, X.; Shi, L.; Ran, X.-Y.; Zhang, L.-N.; Xie, K.-P.; Zhao Y.; Chen, J.; Ye, L.; Yu, X.-Q.; Li, K.* Rational Design of an Intramolecular Hydrogen Bond Enhanced Fluorescent Probe for Diagnosis of Drug-Induced Liver Injury, ACS Materials Lett. 2024, 6, 1059-1068.
18. Zhang, H.; Zhu, G.-N.; Xiang, F.-F.; Chen, Y.-J.; Chen, S.-Y.; Wu, M.*; Li, K.*, High-Throughput Screening of Antioxidant Drug Candidates from Natural Antioxidants with a “Zero” Intrinsic Fluorescence Peroxynitrite Sensing Precursor. J. Med. Chem. 2024, 67, 17855−17865
19. Li, J.-M.; Xiang, F.-F.; Zhou, D.-H.; Xu, J.-X.; Zhang, H.; Liu, Y.-Z.; Kong, Q.-Q.; Yu, X.-Q.; Li, K.* Rational Design of Near-Infrared Fluorescent Probes for Accurately Tracking Lysosomal Viscosity with Allyl Anchoring Si-Rhodamine, Chem. Biomed. Imag. 2024, 2, 126-134.
20. Ran, X.-Y.; Chen, P.; Liu, Y.-Z.; Shi, L.; Chen, X.; Liu, Y.-H.; Zhang, H.; Zhang, L.-N.; Li, K.*; Yu, X.-Q., Rational Design of Polymethine Dyes with NIR-II Emission and High Photothermal Conversion Efficiency for Multimodal Imaging-Guided Photo-immunotherapy, Adv. Mater. 2023, 2210179
21. Liu, Y.‐Z.; Ran, X.-Y.; Zhou, D.-H.; Zhang, H.; Chen, Y.-J.; Xu, J.-X.; Chen, S.-Y.; Kong, Q.-Q.; Yu, X.-Q.; Li, K.* Novel Dibenzofulvene-Based NIR-II Emission Phototheranostic Agent with an 82.6% Photothermal Conversion Efficiency for Photothermal Therapy. Adv. Funct. Mater. 2023, 33, 2311365.
22. Zhang, H.; Xiang, F.-F.; Liu, Y.-Z.; Chen, Y.-J.; Zhou, D.-H.; Liu, Y.-H.; Chen, S.-Y.; Yu X.-Q.; Li, K.* Molecular Engineering of Sulfone-Xanthone Chromophore for Enhanced Fluorescence Navigation, JACS Au, 2023, 3, 3462−3472
23. Chen, Y.-J.; Zhang, H.; Liu, Y.-Z.; Shi, L.; Xiang, F.-F.; Lin, R.-D.; Liu, Y.-H.; Chen, S.-Y.; Yu, X.-Q.; Li, K.*, Rational Design of pH-Independent and High-Fidelity Near-Infrared Tunable Fluorescent Probes for Tracking Leucine Aminopeptidase In Vivo; ACS Sen. 2023, 8, 2359-2367.
24. Liu, Y.-Z.; Zhang, H.; Zhou, D.-H.; Liu, Y.-H.; Ran, X.-Y.; Xiang, F.-F.; Zhang, L.-N.; Chen, Y.-J.; Yu, X.-Q.; Li, K.*, Migration from Lysosome to Nucleus: Monitoring Lysosomal Alkalization-Related Biological Processes with an Aminofluorene-Based Probe. Anal. Chem. 2023, 95, 7294-7302.
25. Zhang, H.; Shi, L.; Li, K.*; Liu, X. Won, M.; Liu, Y.-Z.; Choe, Y.; Liu, X.-Y.; Liu, Y.-H.; Chen, S.-Y.; Yu, K.-K.; Kim, J.-S.*; Yu X.-Q.* Discovery of an Ultra-rapid and Sensitive Lysosomal Fluorescence Lipophagy Process. Angew. Chem. Int. Ed. 2022, e202116439
近五年授权专利：
1.李坤，张丽娜，陈善勇，余孝其，一种pH响应染料平台及其制备方法和应用， 专利号: ZL202311315277.9
2.于抗抗，李坤，雷林，吴思娴，许文明，余孝其， 一种硝基还原酶响应型荧光探针及其制备，专利号: ZL202510264065.5
3.李坤，刘均，向飞帆，李乐雨，陈昱瑾，一种基于硅原子取代的罗丹明衍生物的荧光探针及其制备方法和应用, 专利号: ZL202410754604.9.
4.李坤，陈雪，石磊，陈善勇，余孝其，一种基于嘌呤骨架的聚集诱导发光型光敏剂及其制备方法和应用, 专利号: ZL202410153217.X.
5.吴思娴，许文明，于抗抗; 李坤，余孝其 一种精子中线粒体DNA G-四聚体在男性不育症中的应用和产品，专利号：ZL202310155039.X
6.于抗抗，李坤，余孝其，王浩源 一种基于嘌呤骨架的近红外发射荧光分子及其制备方法和应用，专利号: ZL 202310213757.8
7.李坤，冉小云，陈善勇，余孝其 基于呫吨骨架的近红外II区造影剂及其制备方法和应用，专利号: ZL202211437219.9.
8.李坤，张宏，刘艳红，余孝其, 基于碳原子的罗丹明衍生物骨架的溶酶体靶向染色试剂及其制备方法和应用, 专利号: ZL202011023013.2.
9.李坤，刘艳昭，陈善勇，余孝其， 基于氨基芴骨架的快速及长时间溶酶体或细胞核靶向的近红外染色试剂及其制备方法和应用， 专利号: ZL202111115590.9.
10.李坤，张宏，刘艳红，余孝其， 硅基罗丹明荧光染色试剂及其制备方法和应用， 专利号: ZL202011023009.6.
11.李坤，张宏，陈善勇，余孝其， 基于磷原子取代罗丹明衍生物骨架的染色试剂及其制备方法和应用，专利号: ZL202011022995.3.
12.李坤，石磊，于抗抗，刘艳红，余孝其， 基于嘌呤骨架的聚集诱导型脂滴靶向染色试剂及其制备方法和应用，专利号: ZL202010347324.8.
