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李 坤
职称与职务
教 授、副院长
专      业
化学生物学、有机化学
电      话
85418580
E-Mail
简  历
1999.9-2003.7   烟台大学 化学生物理工学院 学士
2003.9-2008.6   四川大学 化学学院 硕、博士
2008.7- 2011.6  四川大学 化学学院 讲师
2010.11-2011.10 香港大学 化学学院 博士后
2011.7-2016.8   四川大学 化学学院 副教授
2016.9-至今     四川大学 化学学院 教授
主要研究方向
1.反应型荧光探针的设计、合成及在生命体内的应用研究
2.核酸化学生物学
主要工作业绩
主要工作业绩及奖励:
开展以细胞内相关活性物质为导向的荧光分子探针的设计合成及其应用研究。设计合成了一系列基于细胞内活性氧(ROS)活性硫(RSS)和活性氮(RNS)等信号转导分子的荧光探针,实现了快速、高选择及定量细胞内H2S, SO2, ClO-及ONOO-等活性物质的检测,特别针对于线粒体靶向的分子荧光探针开展了深入研究。我们也针对特异性核酸(如G4链体DNA,mRNA等)和含磷分子(ATP, PPi)、等开展了系列研究工作。 已在Chem. Soc. Rev., Biomaterials,Chem. Commun.,等杂志上共发表SCI收录学术论文50多篇,他引1000余次,获准国家发明专利三项。

2010, 2012, 2013 及2014年分别获四川大学“优秀青年骨干教师”称号。

科研项目:
1. 国家自然科学基金面上项目(21572147),DNA动态甲基化过程中5-醛基胞嘧啶选择性反应型小分子荧光探针研究,项目负责人,2016.1-2019.12
2. 四川大学优秀青年学者基金(2013SCU04A41):DNA甲基化过程中胞嘧啶类衍生物的选择性识别,项目负责人,2013.10-2016.12

3. 国家自然科学基金青年项目(21001077),“自活化”序列选择性人工核酸酶的设计合成及应用研究,项目负责人,2011.1-2013.12。
代表性成果 (获奖成果、专著、论文、专利)
代表性成果:

         1. Yang J.; Li, K.*; Hou, J.-T.; Lu C.-Y.; Li, L.-L.; Yu, K.-K.; Yu, X.-Q.* A novel coumarin-based water-soluble fluorescent probe for endogenously generated SO2 in living cells. Sci China Chem, 2017, 60: 793–798.

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         2. Li, K.#; Hou, J.-T.#; Yang, J.; Yu, X.-Q. A tumor-specific and mitochondria-targeted fluorescent probe for real-time sensing of hypochlorite in living cells. Chem. Commun.2017, 53, 5539-5541.

         3.  Hou, J.-T,#; Ren, W.-X.#; Li, K.#; Seo, J.; Sharma, A.; Yu, X.-Q.*; Kim, J.-S.*, “Fluorescent bioimaging of pH: from design to applications” Chem. Soc. Rev.2017, 46, 2076-2090.

         4. Yu, K.-K; Li, K.*; Qin, H.-H.; Zhou, Q.; Qian, C.-H..; Liu, Y.-H.; Yu, X.-Q,* “Construction of pH-sensitive “Submarine” based on gold nanoparticles with double insurance for intracellular pH mapping, quantifying of whole cells and in vivoapplications”. ACS Appl. Mater. Interfaces.2016, 8, 22839?22848. 

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 5. Li, L.-L.; Li, K.*;Liu, Y.-H.; Xu, H.-R. Yu, X.-Q.* “Red emission fluorescent probes for visualization of monoamine oxidase in living cells”. Sci. Rep.2016, 6, 31217.

 6. Yang, J.;Li, K.* ; Hou, J.-T.; Li, L.-L.; Lu, C.-Y.;Xie, Y.-M.: Wang, X.;Yu, X.-Q,* “Novel Tumor-Specific and Mitochondria-Targeted near-Infrared-Emission Fluorescent Probe for SO2 Derivatives in Living Cells”, ACS Sens., 2016, 1, 166-172.

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  7. Liu, Y.; Li, K.*; Xie, K.-X.; Li, L.-L.; Yu, K.-K.; Wang, X.; Yu, X.-Q.* “A water-soluble and fast-response mitochondria-targeted fluorescent probe for colorimetric and ratiometric sensing of endogenously generated SO2derivatives in living cells”, Chem. Commun.2016,52, 3430-3433.

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  8. Chen, X.-L.; Chen, H.; Lu, C.-Y.; Yang, C.; Yu, X.-Q.; Li, K.*; Xie. Y.* “Novel mitochondria-targeted, nitrogen mustard-based DNA alkylation agents with near infrared fluorescence emission”Talanta, 2016, 161, 888-893.

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  9. Ran, Y.-Z.; Xu, H.-R.; Li, K.*; Yu, K.-K.; Yang, J.; Yu, X.-Q.*“Development of a mitochondria-targeted fluorescent probe for hydrazine monitoring in living cells”. RSC Adv.2016, 6, 111016-111019.

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  10. Wu, M.-Y.; Li, K.*; Liu, Y.-H.;Yu, K.-K.; Xie, Y.-M..; Zhou, X.-D.*; Yu, X.-Q.* “Mitochondria-targeted ratiometric fluorescent probe for real time monitoring pH in living cells” Biomaterials2015, 53, 669-678.

  11. Liu, Y.; Li, K.*; Wu, M.-Y.; Liu, Y. -H.; Xie, Y.-M*; Yu, X.-Q.* “A Mitochondria-targeted Colorimetric and Ratiometric Fluorescent Probe for Biological SO2 Derivatives in Living Cells”Chem. Commun.2015, 51,10236-10239.

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  12. Hou, J.-T.; Li, K.*; Yang, J.; Yu, K.-K.; Liao, Y.-X.; Ran, Y.-Z.; Liu, Y.-H., Zhou, X. D.*; Yu, X.-Q.* “A ratiometric fluorescent probe for in situ quantification of basal mitochondrial hypochlorite in cancer cells.”Chem. Commun.2015, 51, 6781-6784.

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  13. Yu, K.-K; Hou, J.-T.; Li, K.*; Yao, Q.; Yang, J.; Wu, M.-Y, Xie, Y.-M.: Yu, X.-Q,* “A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells”Sci. Rep.2015, 5, 15540

  14. Jiao, S.-Y.; Li,K.*; Zhang, W.; Liu, Y.-H.; Huang, Z.; Yu,X.-Q.*“Cd(II)-terpyridine-based complex as a ratiometric fluorescent probe for pyrophosphate detection in solution and as an imaging agent in living cells.”Dalton Trans., 2015,44, 1358-1365.

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  15. Jiao, S.-Y.; Li K.*Wang, X.; Huang, Z.; Pu, L.; Yu, X.-Q.* “Making pyrophosphate visible: the first precipitable and real-time fluorescent sensor for pyrophosphate in aqueous solution.” Analyst, 2015, 140, 174–181.

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  16. Hou, J.-T.; Yang, J.; Li, K.*; Yu, K.-K.;Yu, X.-Q.*“A colorimetric and red emissive fluorescent probe for cysteine and its application in bioimaging.”Sensor Actuat. B: Chem., 2015, 214, 92-100.

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  17. Liao, Y.-X.; Wang, M.-D.;Li, K,*; Yang, Z.-X.; Hou, J.-T.; Wu,M.-Y.; Liu, Y.-H.; Yu, X. Q.*“A highly sensitive and selective “turn-on” fluorescent probe for hypochlorous acid monitoringRSC Adv., 2015, 5, 18275-18278.

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  18. Xu, H.-R.;Li, K.*; Jiao, S.-Y.; Pan, S.-L.; Zeng, J.-R.; Yu, X.-Q.*“Tetraphenylethene–pyridine salts as the firstself-assembling chemosensor for pyrophosphate”Analyst, 2015, 140, 4182-4188.

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  19. Xu, H.-R.;Li, K.*; Jiao, S.-Y.; Li, L.-L.; Pan, S.-L.; Yu, X.-Q.*Tetraphenylethene based zinc complexes as fluorescent chemosensorsfor pyrophosphate sensing”, Chin. Chem. Lett.2015, 26, 877-880.

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         20. Xu, H.-R.; Li, K. *; Wang, M.-Q.; Wang, B.-L.; Wang, X.; Yu, X.-Q.* The dicyclen–TPE zinc complex as a novel fluorescent ensemble for nanomolar pyrophosphate sensing in 100% aqueous solution. Org. Chem. Front., 2014, 1, 1276–1279.

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  21. Hou,J.-T.;Yang, J.; Li,K.*; Liao, Y.-X.; Yu,K.-K.;Xie, Y.-M.; Yu, X.-Q.*A highly selective water-soluble optical probe forendogenous peroxynitrite.Chem. Commun. 2014, 50, 9947-9950.

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  22. Hou,J.-T.; Wu, M.-Y. Li,K.*Yang, J.; Yu,K.-K.; Xie, Y.-M.*; Yu, X.-Q.* Mitochondria- targeted colorimetric and fluorescent probes for hypochlorite and their applications for in vivo imaging.Chem. Commun. 2014, 50, 8640-8643.

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  23. Wu, M.-Y.; Li, K.*; Li, C.-Y.; Hou, J.-T.; Yu, X.-Q.* A Water-Soluble Near-Infrared Probe for Colorimetric and Ratiometric Sensing of SO2 Derivatives in Living Cells. Chem. Commun. 2014,50, 183-185.

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  24. Yu,K.-K.;Li,K.*Hou,J.-T.;Yang, J.;Xie, Y.-M.*; Yu, X.-Q.*Rhodamine based pH-sensitive “intelligent” polymer as lysosome targeting probes and their imaging application in vivo. Polym. Chem., 2014, 5(19), 5804-5812.

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  25. Yu,K.-K.;Li,K.*Hou,J.-T.;Qin, H.-H;Xie, Y.-M.;Qian, C.-H.; Yu, X.-Q.*Rodamine-based lysosome-targeted fluorescence probes: high pH sensitivity and their imaging application in living cells. RSC Adv., 2014, 4(64), 33975-33980.

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  26. Liao, Y.-X. Li, K.*, Wu, M.-Y.; Wu, T.; Yu, X.-Q.* A selenium-contained aggregation-induced “turn on” fluorescent probe for hydrogen peroxide. Org. Biomol. Chem.2014, 12, 3004-3008.

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  27. He, T.;Li, K.*; Wang, N.; Liao, Y.-X.; Wang, X.; Yu X.-Q * A ferrocene-based multiple-stimuli responsive organometallogel. Soft Matter. 2014,10,3755-3761.

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  28. He,T.; Li,K.*; Wu, M.Y.; Liao, Y.-X.; Yu, X.-Q.* Visual detection of amino acids by supramolecular gel collapse. RSC Adv., 2014, 4, 2119-2123.

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  29. Hou,J.-T.;Li,K.* Yu,K.-K.; Ao,M.-Z.; Wang, X.; Yu, X.-Q.* Novel triazole-based fluorescent probes for Pd2+ inaqueous solutions: design, theoretical calculations andImaging. Analyst, 2013, 138, 6632–6638

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  30. Wu, M.-Y.; He, T.; Li, K.*; Wu, M.-B.; Huang, Z.; Xie, Y.-M.*; Yu, X.-Q.*A real-time colorimetric and ratiometric fluorescent probe for sulfite. Analyst.2013, 138(10), 3018-3025.

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  31. Xu, H.-R.; Li, K.*; Liu, Q.; Wu, T.-M.; Wang, M.-Q.; Hou, J.-T.; Huang, Z.; Xie, Y.-M.*; Yu, X.-Q.*Dianthracene-cyclen conjugate: the first equal-equivalent responding fluorescent chemosensor for Pb2+ in aqueous solution.Analyst2013, 138(8), 2329-2334.

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  32. Hou, J.-T.; Li, K.*; Yu, K.-K.; Wu, M.-Y.; Yu, X.-Q.* “Coumarin-DPA-Cu(II) as a chemosensing ensemble towards histidine determination in urine and serum” Org. Biomol. Chem. 2013, 11(5), 717-720.

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  33. Hou, J.-T.; Liu, B.-Y. ; Li K.* ; Yu K.-K.; Wu, M.-B.; Yu, X.-Q.* Two birds with one stone: Multifunctional and highly selective fluorescent probe for distinguishing Zn2+ from Cd2+and selective recognition of sulfide anion.Talanta,2013,116, 434–440.

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  34. Jiao, S.-Y.; Peng, L.-L.;Li, K.*; Xie, Y.-M.; Ao, M.-Z.; Wang, X.*; Yu, X.-Q.*  A BINOL-based ratiometric fluorescent sensor for Zn2+ and in situ generated ensemble for selective recognition of histidine in aqueous solution. Analyst, 2013, 138, 5762-5768.

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  35. Li, K.; Xu, H.-R.; Yu, K.-K.; Hou, J.-T.; Yu, X.-Q.* “A coumarin-based chromogenic and ratiometric probe for hydrazineAnal. Methods2013, 5, 2653-2656.

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  36. Hou, J.-T.; Li, K.*; Liu, B.-Y.; Liao, Y.-X.; Yu, X.-Q.* “The first ratiometric probe for lysine in water” Tetrahedron 2013, 69(9), 2118-2123.

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  37. Wang, M.-Q.; Li K.*; Xu, H.-R.; Yu X.-Q.* Imidazolium-based 1,1′-bi-2-naphthol fluorescent probe for ratiometric and selective detection of DNA in water. Anal. Methods2013, 5, 5903-5907

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  38. Yu,K.-K.; Li, K.*;Hou,J.-T.;Yu, X.-Q.*Coumarin–TPA derivative: a reaction-based ratiometric fluorescent probe for Cu(I). Tetrahedron Lett.2013, 54, 5771–5774

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  39. Wang,M.-Q.;Li K.*;HouJ.-T.;Wu, M.-Y., Huang, Z.; Yu X.-Q.* “BINOL-Based Fluorescent Sensor for Recognition of Cu(II) and Sulfide Anion in Water” J. Org. Chem.2012, 77, 8350?8354

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  40. Wu, M.-Y., Li, K.*;Hou, J.-T.; Huang, Z.; Yu, X.-Q.* A selective colorimetric and ratiometric fluorescent probe for hydrogen sulfide.Org. Biomol. Chem. 2012, 10, 8342-8347.

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专利

    1. 余孝其,李坤,吴明雨,王娜,一种合成4-芳基-45-二氢呋喃类衍生物的新方法。专利号:ZL 201010160246.7授权日期: 20121212

    2. 余孝其,李坤,后际挺取代的水杨醛-TCF衍生物及其制备方法和用途。专利号: ZL201410023272.3 授权日期: 2015114

 

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